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Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Pricing & Availability
Clone
OX-7 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy-1.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_OX-7_Alx647_021308
LOU rat thymocytes stained with OX-7 Alexa Fluor® 647
  • A_OX-7_Alx647_021308
    LOU rat thymocytes stained with OX-7 Alexa Fluor® 647
  • B_OX-7_A647_CD90_CD90point1_IHCF_101218
    FVB/N mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-rat CD90/mouse CD90.1 (clone OX-7) Alexa Fluor® 647 (red) overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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202507 25 µg 81€
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202508 100 µg 184€
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat, Mouse
Reported Reactivity
Rabbit, Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte Thy-1 antigen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
  2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
  3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
  4. Eitner F. 1997. Kidney. Int. 51:69.
  5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
  6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
  8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
  9. Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
  10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)
Product Citations
  1. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  2. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  3. Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed
  4. Kameishi S, et al. 2023. Sci Rep. 13:4421. PubMed
  5. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  6. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
  7. Yamada T, et al. 2016. J Immunol. 197: 4079 - 4089. PubMed
  8. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  9. Young R, et al. 2009. Blood. 114:5016. PubMed
  10. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  11. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  12. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  13. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  14. Brede C, et al. 2012. J Clin Invest. 122:4439. PubMed
RRID
AB_492885 (BioLegend Cat. No. 202507)
AB_492885 (BioLegend Cat. No. 202508)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

Function
Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
Interacts with CD45
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID
21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt
View information about CD90/CD90.1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD90/CD90.1 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Purified anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F,ICC,IHC-P,IP,WB
FITC anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 488 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F
Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PerCP anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PE anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Biotin anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 650™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 510™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 711™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PE/Dazzle™ 594 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 IHC-F
TotalSeq™-A0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
TotalSeq™-B0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
TotalSeq™-C0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
Brilliant Violet 785™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Go To Top Version: 5    Revision Date: 12.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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