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Alexa Fluor® 647 anti-rat IgG2b Antibody

Pricing & Availability
Clone
MRG2b-85 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Mouse IgG1, κ
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Product Citations
publications
a-MRG2b-85_A647_IgG2b_Antibody_IHC_070617
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD3 (clone 17A2) purified and 10 µg/ml of B220 (Clone RA3-6B2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2b (Clone MRG2b-85) Alexa Fluor® 647 (red) for 2 hours at room temperature. The image was captured by 10X objective.
  • a-MRG2b-85_A647_IgG2b_Antibody_IHC_070617
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD3 (clone 17A2) purified and 10 µg/ml of B220 (Clone RA3-6B2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG2b (Clone MRG2b-85) Alexa Fluor® 647 (red) for 2 hours at room temperature. The image was captured by 10X objective.
  • b-MRG2b-85_A647_IgG2b_Antibody_ICC_2_062123
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with MeOH for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then stained with 5.0 µg/mL of purified anti-eIF4B (Cat. No. 617702) overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-rat IgG2b (clone MRG2b-85) at 2.5 µg/mL. Nuclei were counterstained with DAPI (panel A) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar = 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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408208 25 µg 85€
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408209 100 µg 160€
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Description

The MRG2b-85 monoclonal antibody reacts with rat immunoglobulin G2b (IgG2b) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). The MRG2b-85 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
RRID
AB_2715885 (BioLegend Cat. No. 408208)
AB_2715885 (BioLegend Cat. No. 408209)

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Go To Top Version: 2    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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