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APC anti-human CD13 Antibody

Pricing & Availability
Clone
WM15 (See other available formats)
Regulatory Status
RUO
Workshop
IV M44
Other Names
Aminopeptidase N, APN, gp150
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM15APC
Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM15 APC
  • WM15APC
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM15 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
301705 25 tests 91€
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301706 100 tests 215€
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Description

CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cotton-topped Tamarin
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Saiki I, et al. 1993. Int J Cancer. 54:137. (Block)
  3. Rosenzwajg M, et al. 2000. Blood 95:453. (Block)
  4. Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
  5. Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)
Product Citations
  1. He Y, et al. 2023. Protein Cell. 14:202. PubMed
  2. Liang C, et al. 2021. Cell Res. 31:187. PubMed
  3. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  4. Pfaender S, et al. 2020. Nat Microbiol. 1.131944444. PubMed
  5. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  6. Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed
  7. Fu L, et al. 2019. PLoS Biol. 17:e3000201. PubMed
  8. Yan X, et al. 2019. Oncol Rep. 41:1938. PubMed
  9. Deng Z, et al. 2021. Mol Med Rep. :23. PubMed
  10. Silva G, et al. 2013. PLoS One. 8:e53766. PubMed
  11. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
  12. Thiel A, et al. 2015. Sci Rep. 5: 17685. PubMed
RRID
AB_314181 (BioLegend Cat. No. 301705)
AB_314181 (BioLegend Cat. No. 301706)

Antigen Details

Structure
Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
Distribution

Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset

Function
Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
Ligand/Receptor
Coronavirus receptor
Cell Type
Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Shipp M, et al. 1993. Blood 82:1052.
2. Larsen S, et al. 1996. J. Exp. Med. 184:183.

Gene ID
290 View all products for this Gene ID
UniProt
View information about CD13 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03.21.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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