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APC anti-human CD338 (ABCG2) Antibody

Pricing & Availability
Clone
5D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
ABCG2, CDw338, Bcrp1, MXR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
5D3_APC_092308.jpg
Human ABCG2 transfected cells stained with 5D3 APC
  • 5D3_APC_092308.jpg
    Human ABCG2 transfected cells stained with 5D3 APC
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332020 100 tests 252€
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Description

CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34.  In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells.  The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells.  The expression of ABCG2 appears to be highly conserved as it has been identified in various species.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

This clone is listed under the following US Patent Numbers:
6,313,277; 7,541,437; 7,655,755; 7,741,455

Application References

(PubMed link indicates BioLegend citation)
  1. Matalia H, 2012. Br J Ophthalmol. PubMed
  2. 2. Zhou S, et al. 2001. Nat. Med. 7:1028. (FC)
  3. Abbott BL, et al. 2002. Blood 100:4594. (FC)
  4. Huang FF, et al. 2104. PloS One. 9:88298. PubMed
Product Citations
  1. Shetty R, et al. 2015. Br J Ophthalmol. 99:272. PubMed
  2. Moiseeva NI, et al. 2022. Int J Mol Sci. 23:. PubMed
  3. Matalia H, et al. 2012. Br J Ophthalmol. 96:1339. PubMed
  4. Raman S, et al. 2021. Cell Reports Medicine. 2(7):100344. PubMed
  5. Prasanphanich A, et al. 2016. PLoS Comput Biol. 12:e1005188. PubMed
RRID
AB_1227470 (BioLegend Cat. No. 332020)

Antigen Details

Distribution

primitive 'sidepopulation' (SP) stem cells, KDR+ human stem cells

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Kim M, et al. 2002. Clin. Cancer Res. 8:228.
2. Scharenber CW, et al. 2002. Blood 99:50712.
3.Goodell MA, et al. 1997. Nat. Med. 3:133745.
4. Bunting KD. 2002. Stem Cells. 20:11.

Gene ID
9429 View all products for this Gene ID
UniProt
View information about CD338 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.17.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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