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APC anti-human CD44 Antibody

Pricing & Availability
Clone
BJ18 (See other available formats)
Regulatory Status
RUO
Workshop
VI A034
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BJ18_APC_011409.jpg
Human peripheral blood lymphocytes stained with BJ18 APC
  • BJ18_APC_011409.jpg
    Human peripheral blood lymphocytes stained with BJ18 APC
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338805 25 tests 120€
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338806 100 tests 280€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Normal human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.
Product Citations
  1. Delle Cave D, et al. 2022. J Exp Clin Cancer Res. 41:315. PubMed
  2. Liu X, et al. 2023. Nat Commun. 14:238. PubMed
  3. Calvanese V, et al. 2022. Nature. 604:534. PubMed
  4. Wang C, et al. 2020. British Journal of Pharmacology. 177(13):3009-3023. PubMed
  5. Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed
  6. Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
  7. Deshmukh A, et al. 2018. Cancers (Basel). 10. PubMed
  8. Wang X, et al. 2020. Front Oncol. 1.083333333. PubMed
  9. Parker KA, et al. 2021. Mol Cancer Res. 19:2068. PubMed
  10. Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed
  11. Ragni E, et al. 2022. Biomolecules. 12:. PubMed
  12. Hyun Hee Lee, Vanessa Bellat, Benedict Law 2017. PLoS One. 12(2):e0171044. PubMed
  13. Jang G, et al. 2022. NPJ Genom Med. 7:63. PubMed
  14. Bryson BL, et al. 2021. Mol Cancer Res. 19:651. PubMed
  15. Bugalho A, et al. 2013. Clin Lung Cancer. 14:704. PubMed
  16. Adnani L, et al. 2022. Nat Commun. 13:5494. PubMed
  17. Kim M, et al. 2017. Cytokine. 10.1016/j.cyto.2017.07.007. PubMed
  18. Grayson KA, et al. 2021. PLoS One. 16:e0246733. PubMed
  19. Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed
  20. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  21. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
  22. Raman S, et al. 2021. Cell Reports Medicine. 2(7):100344. PubMed
  23. Beghelli D, et al. 2017. Oxid Med Cell Longev. 10.1155/2017/7468064. PubMed
  24. Luo HT, et al. 2021. NPJ Genom Med. 6:48. PubMed
  25. Lee CH, et al. 2018. Elife. 7:e32532. PubMed
  26. Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
  27. Repas J, et al. 2021. Sci Rep. 11:21354. PubMed
RRID
AB_1501195 (BioLegend Cat. No. 338805)
AB_1501195 (BioLegend Cat. No. 338806)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.

Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org
Go To Top Version: 2    Revision Date: 07.18.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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