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APC anti-human GARP (LRRC32) Antibody

Pricing & Availability
Clone
7B11 (See other available formats)
Regulatory Status
RUO
Other Names
Garpin, Glycoprotein A repetitions predominant
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
7B11_APC_1_090111
Human peripheral blood mononuclear cells were stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24 hours; and then stained with CD4 PerCP, LAP (TGF-β) PE and GARP (7B11) APC (top) or mouse IgG2b, κ APC isotype control (bottom). Data was analyzed by gating on CD4-positive cells.
  • 7B11_APC_1_090111
    Human peripheral blood mononuclear cells were stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24 hours; and then stained with CD4 PerCP, LAP (TGF-β) PE and GARP (7B11) APC (top) or mouse IgG2b, κ APC isotype control (bottom). Data was analyzed by gating on CD4-positive cells.
  • 7B11_APC_2_090111
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352505 25 tests 129€
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352506 100 tests 288€
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Description

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LRRC32-DNA vaccination
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Eschborn M, et al. 2015. J Allergy Clin Immunol. 136: 159-168. PubMed
  2. Chaumonnot K, et al. 2021. Cell Death Dis. 12:114. PubMed
  3. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
RRID
AB_10896059 (BioLegend Cat. No. 352505)
AB_10896059 (BioLegend Cat. No. 352506)

Antigen Details

Structure
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution

Activated Tregs, megakaryocytes, platelets

Ligand/Receptor
LAP (TGF-β1)
Cell Type
Megakaryocytes, Platelets, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.

Gene ID
2615 View all products for this Gene ID
UniProt
View information about GARP on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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