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APC anti-mouse H-2Kb bound to SIINFEKL Antibody

Pricing & Availability
Clone
25-D1.16 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype
Mouse IgG1, κ
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Product Citations
publications
25-D1.16_APC_052412
C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hrs, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) APC (purple histogram indicates pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ APC isotype control (black open histogram).
  • 25-D1.16_APC_052412
    C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hrs, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) APC (purple histogram indicates pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ APC isotype control (black open histogram).
  • 25-D1.16_APC_H2Kb-SIINFEKL_Antibody_IF_092012
    CHO cells expressing inducible single chain H-2Kb-SIINFEKL construct were induced by the addition of doxycycline overnight. The negative control is non-doxycycline treated cells, same staining procedure. Stained live cells in PBS+1%NGS+ APC-H-2Kb-SIINFEKL, clone 25-D1.16 (1:200 dilution, 10 µg/ml) for 1h RT, then fixed with PFA 4% RT 10 min and counterstained with DAPI 1 mg/ml RT 5 min. Image courtesy of Dr. Javier Casas, The Scripps Research Institute.
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141605 25 µg 91€
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141606 100 µg 280€
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Description

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SIINFEKL pulsed RMA-S cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Application References

(PubMed link indicates BioLegend citation)
  1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 
  2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
  3. Porgador A, et al. 1997. Immunity 6:715. (IF, IHC)
  4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
Product Citations
  1. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  2. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  3. Malka Y, et al. 2022. Mol Cell. 82:3840. PubMed
  4. Li J, et al. 2022. Elife. 11:. PubMed
  5. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  6. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  7. Hao H, et al. 2023. Nat Biomed Eng. . PubMed
  8. Champagne J, et al. 2021. Mol Cell. 81:4709. PubMed
  9. Yan J, et al. 2022. J Clin Invest. 132:. PubMed
  10. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  11. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Ylösmäki E, et al. 2021. J Immunother Cancer. 9:. PubMed
  13. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  14. Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed
  15. Bartok O, et al. 2020. Nature. . PubMed
  16. Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed
  17. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  18. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  19. Song TY, et al. 2021. Nat Commun. 12:7003. PubMed
  20. Cordeiro B, et al. 2020. Cell Reports. 31(5):107585. PubMed
  21. Zhang Y, et al. 2022. Nat Commun. 13:4948. PubMed
  22. Reguzova A, et al. 2020. Vaccines (Basel). 8:. PubMed
  23. Ahn J et al. 2018. Cancer cell. 33(5):862-873 . PubMed
  24. Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed
RRID
AB_11219402 (BioLegend Cat. No. 141605)
AB_11219402 (BioLegend Cat. No. 141606)

Antigen Details

Structure
OVA peptide257-264 and H-2Kb complex
Distribution

SIINFEKL bond H-2Kb bearing cells

Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb bound to SIINFEKL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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