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APC/Cyanine7 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_APCCyanine7_NK11_Antibody_1_022324
C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
  • PK136_APCCyanine7_NK11_Antibody_1_022324
    C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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108723 25 µg 76€
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108724 100 µg 234€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  3. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  4. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  5. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  6. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  7. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  8. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  9. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  10. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
  11. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  12. Qiao Y, et al. 2021. Oncoimmunology. 10:1947569. PubMed
  13. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  14. Mitchell A, et al. 2012. J Immunol. 189:4970. PubMed
  15. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  16. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  17. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  18. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  19. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  20. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  21. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  22. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  23. Li J, et al. 2018. Immunity. 49:178. PubMed
  24. Brodin P, et al. 2010. PLoS One. 5:e13174. PubMed
  25. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  26. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  27. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  28. Goossens P, et al. 2019. Cell Metab. 29:1376. PubMed
  29. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  30. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  31. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  32. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  33. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  34. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  35. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  36. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  37. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  38. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  39. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  40. Barth ND, et al. 2018. J Immunol. 201:2602. PubMed
  41. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  42. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  43. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  44. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  45. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  46. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  47. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  48. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  49. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  50. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  51. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  52. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  53. Etzerodt A, et al. 2020. J Exp Med. 217:00:00. PubMed
  54. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  55. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_830870 (BioLegend Cat. No. 108723)
AB_830870 (BioLegend Cat. No. 108724)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 2    Revision Date: 12.08.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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