APC/Fire™ 810 anti-human CD45RO, CD45RO, UCHL1

Pricing & Availability

Clone: UCHL1 (See other available formats)
Regulatory Status: RUO
Workshop: IV N31
Other Names: CD45RO
Isotype: Mouse IgG2a, κ
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Product Citations: publications

UCHL1_APC-FIRE-810_CD45RO_FC_031924 — Human peripheral blood lymphocytes were stained with anti-human CD45RA (clone HI100) PE and anti-human CD45RO (clone UCHL1) APC/Fire™ 810 (left) or mouse IgG2a, κ APC/Fire™ 810 isotype control (right).

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304273

25 tests

141€

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304274

100 tests

306€

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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cynomolgus, Common Marmoset
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IL-2 dependent T cell line, CA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁵ and formalin-fixed paraffin-embedded tissue sections⁴, Western blotting², and immunoprecipitation³.
RRID: AB_3106124 (BioLegend Cat. No. 304273)
AB_3106124 (BioLegend Cat. No. 304274)

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution: Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: CD22
Cell Type: B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RO on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD45RO Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD45RO	UCHL1	FC
FITC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC,SB
PE/Cyanine5 anti-human CD45RO	UCHL1	FC
Purified anti-human CD45RO	UCHL1	FC,WB,IP,IHC-P,SB
Alexa Fluor® 488 anti-human CD45RO	UCHL1	FC,IHC-P
Pacific Blue™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 700 anti-human CD45RO	UCHL1	FC
Biotin anti-human CD45RO	UCHL1	FC
Brilliant Violet 421™ anti-human CD45RO	UCHL1	FC,IHC-P
PerCP/Cyanine5.5 anti-human CD45RO	UCHL1	FC
Brilliant Violet 570™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 605™ anti-human CD45RO	UCHL1	FC
APC/Cyanine7 anti-human CD45RO	UCHL1	FC
PE/Cyanine7 anti-human CD45RO	UCHL1	FC
Brilliant Violet 650™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 711™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 785™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 594 anti-human CD45RO	UCHL1	IHC-P
Purified anti-human CD45RO (Maxpar® Ready)	UCHL1	FC,CyTOF®
Brilliant Violet 510™ anti-human CD45RO	UCHL1	FC
PE/Dazzle™ 594 anti-human CD45RO	UCHL1	FC
APC/Fire™ 750 anti-human CD45RO	UCHL1	FC
PerCP anti-human CD45RO	UCHL1	FC
APC anti-human CD45RO	UCHL1	FC
TotalSeq™-A0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-B0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-C0087 anti-human CD45RO	UCHL1	PG
Brilliant Violet 750™ anti-human CD45RO	UCHL1	FC
PE/Fire™ 640 anti-human CD45RO	UCHL1	FC
TotalSeq™-D0087 anti-human CD45RO	UCHL1	PG
Spark Violet™ 423 anti-human CD45RO	UCHL1	FC
GMP APC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC
TotalSeq™-Bn1369 anti-human CD45RO	UCHL1	SB
GMP PE anti-human CD45RO	UCHL1	FC
Spark UV™ 387 anti-human CD45RO	UCHL1	FC
APC/Fire™ 810 anti-human CD45RO	UCHL1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.19.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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