APC/Fire™ 750 anti-human CD43 Antibody, CD43, CD43-10G7

Pricing & Availability

Clone: CD43-10G7 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Isotype: Mouse IgG1, κ
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CD43-10G7_APCFire750_CD43_Antibody_1_021524 — Human lysed whole blood was stained with anti-human CD43 (clone CD43-10G7) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).

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343219

25 tests

141€

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343220

100 tests

306€

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Description

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MV4-11 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3106031 (BioLegend Cat. No. 343219)
AB_3106031 (BioLegend Cat. No. 343220)

Antigen Details

Structure: Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.
Distribution: Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.
Ligand/Receptor: CD54, Siglec-1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6:4029.
3. Thurman EC, et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 6693 View all products for this Gene ID
UniProt: View information about CD43 on UniProt.org

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Other Formats

View All CD43 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD43	CD43-10G7	FC,IP
PE anti-human CD43	CD43-10G7	FC
APC anti-human CD43	CD43-10G7	FC
PE/Cyanine7 anti-human CD43	CD43-10G7	FC
TotalSeq™-A0357 anti-human CD43	CD43-10G7	PG
TotalSeq™-D0357 anti-human CD43	CD43-10G7	PG
TotalSeq™-C0357 anti-human CD43	CD43-10G7	PG
TotalSeq™-B0357 anti-human CD43	CD43-10G7	PG
PE/Fire™ 640 anti-human CD43	CD43-10G7	FC
APC/Fire™ 750 anti-human CD43	CD43-10G7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.15.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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