Biotin anti-Cytochrome c Antibody anti-Cytochrome c

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Clone: 6H2.B4 (See other available formats)
Regulatory Status: RUO
Other Names: Cyt c
Isotype: Mouse IgG1, κ
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Product Citations: publications

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612303

100 µg

175€

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Description

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Rat, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat cyt c-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry⁵, immunofluorescence microscopy^3,5, immunoprecipitation⁴, and immunocytochemistry⁵.
RRID: AB_315776 (BioLegend Cat. No. 612303)

Antigen Details

Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: 1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
Gene ID: 1355 View all products for this Gene ID
UniProt: View information about Cytochrome c on UniProt.org

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Related Protocols

Immunoprecipitation Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Cytochrome c Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-Cytochrome c	6H2.B4	ICFC
FITC anti-Cytochrome c	6H2.B4	ICFC
Purified anti-Cytochrome c	6H2.B4	IP,ICC,ICFC
Alexa Fluor® 488 anti-Cytochrome c	6H2.B4	ICFC
Alexa Fluor® 647 anti-Cytochrome c	6H2.B4	ICFC,ICC
FITC anti-Cytochrome c	6H2.B4	FC
GMP FITC anti-Cytochrome c	6H2.B4	FC
TotalSeq™-B0043 anti-Cytochrome c	6H2.B4	ICPG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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