- Clone
- ITEM-4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fibroblast growth factor-inducible 14 kD protein (Fn14), TWEAK receptor, TNFRSF12A
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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314103 | 25 µg | 104€ |
CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Fn14 transfected P815
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting3,4, immunofluorescence3,4, and immunohistochemistry of frozen sections5 and formalin-fixed paraffin-embedded sections3. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Polek TC, et al. 2003. J. Biol. Chem. 278:32317.
- Nakayama M, et al. 2003. J. Immunol. 170:341.
- Karaca G, et al. 2014. PLoS One. 9:83987. (IHC, IF, WB) PubMed
- Muñoz-García B, et al. 2006. Stroke. 37:2044. (IF, WB)
- Jakubowski A, et al. 2005. J. Clin. Invest. 115:2955. (IHC)
- Karaca G, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308:325. PubMed
- RRID
-
AB_2205623 (BioLegend Cat. No. 314103)
Antigen Details
- Structure
- Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
- Distribution
- Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC
- Function
- Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
- Ligand/Receptor
- TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455. - Gene ID
- 27279 View all products for this Gene ID 51330 View all products for this Gene ID
- UniProt
- View information about CD266 on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All CD266 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human/mouse CD266 (Fn14, TWEAK R) | ITEM-4 | FC,ICC,IHC-P,IHC-F,WB |
Biotin anti-human/mouse CD266 (Fn14, TWEAK R) | ITEM-4 | FC |
PE anti-human/mouse CD266 (Fn14, TWEAK R) | ITEM-4 | FC |
APC anti-human/mouse CD266 (Fn14, TWEAK R) | ITEM-4 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with purified ITEM-4, f... -
Biotin anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with biotinylated ITEM-... -
PE anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with ITEM-4 PE -
APC anti-human/mouse CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with CD266 (clone ITEM-...
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