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Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody

Pricing & Availability
Clone
2E2 (See other available formats)
Regulatory Status
RUO
Other Names
IFN-γRα , IFN-γ Receptor α chain, CDw119
Isotype
Armenian Hamster IgG
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Product Citations
publications
2E2
C57BL/6 splenocytes stained with purified 2E2, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
  • 2E2
    C57BL/6 splenocytes stained with purified 2E2, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
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112803 50 µg 67€
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112804 500 µg 235€
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Description

CDw119 is a 90 kD immunoglobulin superfamily member, also known as IFN-γRα chain. It is a class II cytokine receptor family member that serves as a IFN-γ-binding chain associated with the IFN-γ β chain also known as AF-1. In addition to ligand binding, CDw119 participates in ligand trafficking. CDw119 is expressed on T and B cells, NK cells, fibroblasts, endothelial, and epithelial cells. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Soluble recombinant mouse IFN-γR α chain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112804) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bach EA, et al. 1995. Science 270:1215.
Product Citations
  1. Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed
  2. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  3. Bruin A, et al. 2010. Blood. 116:2559. PubMed
  4. Cella M, et al. 2010. Proc Natl Acad Sci U S A. 107:10961. PubMed
  5. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  6. DanDan Huang, Lang Bao 2016. J Microbiol Immunol Infect. 49:859-865. PubMed
  7. Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
RRID
AB_2123475 (BioLegend Cat. No. 112803)
AB_2123475 (BioLegend Cat. No. 112804)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
Distribution

T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells

Function
Initiates and regulates immune response
Ligand/Receptor
IFN-γ
Cell Type
B cells, Endothelial cells, Epithelial cells, Fibroblasts, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197.

Gene ID
15979 View all products for this Gene ID
UniProt
View information about CD119 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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