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Brilliant Violet 510™ anti-human CD146 Antibody

Pricing & Availability
Clone
P1H12 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Muc-18, MCAM, Mel-CAM, S-endo
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1H12_BV510_CD146_Antibody_100818
Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
  • P1H12_BV510_CD146_Antibody_100818
    Human cervical cancer cell line, HeLa, was stained with CD146 (clone P1H12) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability Save
361021 25 tests 155€
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361022 100 tests 340€
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Description

CD146 is a 118 kD integral transmembrane glycoprotein that is also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Dog, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human umbilical cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: Western blotting3 and IHC1,5. 

Clone P1H12 cross-reacts to Dog2, 5.

Application References

(PubMed link indicates BioLegend citation)
  1. Solovey A, et al. 1997. N. Engl. J. Med. 337:1584. (FC, IHC)
  2. Lamerato-Kozicki AR, et al. 2006. Exp. Hematol. 34:870. (FC)
  3. Balint K, et al. 2005. J. Clin. Invest. 115:3166. (WB)
  4. Neskey DM, et al. 2008. J. Exp. Clin. Cancer Res. 27:61. (ELISA)
  5. Kamstock D, et al. 2006. Cancer Gene Therap. 13:306. (IHC)
RRID
AB_2783269 (BioLegend Cat. No. 361021)
AB_2783269 (BioLegend Cat. No. 361022)

Antigen Details

Structure
118 kD integral glycoprotein, Ig superfamily
Distribution

Melanoma cells, epithelial cells, endothelial cells, activated T cells, multipotent mesenchymal stromal cells

Function
Adhesion, monocytes transendothelial migration
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Pickl WF, et al. 1997. J. Immunol. 158:2107.
2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219.
3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.

Gene ID
4162 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org
Go To Top Version: 2    Revision Date: 12.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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