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Brilliant Violet 510™ anti-human CD83 Antibody

Pricing & Availability
Clone
HB15e (See other available formats)
Regulatory Status
RUO
Other Names
HB15
Isotype
Mouse IgG1, κ
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Product Citations
publications
HB15e_BV510_CD83_Antibody_070623
Human monocyte-derived mature dendritic cells (induced with GM-CSF, IL-4, and TNF-α) were stained with anti-human CD206 PE and anti-human CD83 (clone HB15e) Brilliant Violet 510™ (left) or with mouse IgG1, κ Brilliant Violet 510™ isotype control (right).
  • HB15e_BV510_CD83_Antibody_070623
    Human monocyte-derived mature dendritic cells (induced with GM-CSF, IL-4, and TNF-α) were stained with anti-human CD206 PE and anti-human CD83 (clone HB15e) Brilliant Violet 510™ (left) or with mouse IgG1, κ Brilliant Violet 510™ isotype control (right).
Compare all formats See Brilliant Violet 510™ spectral data
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305347 25 tests 155€
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305348 100 tests 326€
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Description

CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Zhou L, et al. 1995. J. Immunol. 154:3821.
  3. Cao W, et al. 2005. Biochem. J. 385:85.
  4. Lore K, et al. 2002. AIDS 16:683. (IHC)
  5. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
RRID
AB_3068175 (BioLegend Cat. No. 305347)
AB_3068175 (BioLegend Cat. No. 305348)

Antigen Details

Structure
Ig superfamily, single chain transmembrane glycoprotein, 43 kD
Distribution

Dendritic cells, Langerhan cells, activated B and T cells

Cell Type
B cells, Dendritic cells, Langerhans cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kozlow E, et al. 1993. Blood 81:454.
2. Zhou L, et al. 1992. J. Immunol. 149:735.
3. Zhou L, et al. 1995. Blood 86:3295.

Gene ID
9308 View all products for this Gene ID
UniProt
View information about CD83 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.06.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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