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Brilliant Violet 650™ anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
MIH18 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
B7-DC, PD-L2, PDL2, B7DC
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH18_BV650_CD273_Antibody_091323
Human monocyte-derived dendritic cells were stained with anti-human CD11c FITC and anti-human CD273 (B7-DC, PD-L2) (clone MIH18) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right).
  • MIH18_BV650_CD273_Antibody_091323
    Human monocyte-derived dendritic cells were stained with anti-human CD11c FITC and anti-human CD273 (B7-DC, PD-L2) (clone MIH18) Brilliant Violet 650™ (left) or mouse IgG1, κ Brilliant Violet 650™ isotype control (right).
Compare all formats See Brilliant Violet 650™ spectral data
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345529 25 tests 140€
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345530 100 tests 324€
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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. Clone MIH18 is reported to block PDL2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7-DC transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).

Application References

(PubMed link indicates BioLegend citation)
  1. Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
  2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
  3. Hobo W, et al. 2010. Blood 25:4501. (FC)
  4. Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
  5. Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
  6. Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
RRID
AB_3097226 (BioLegend Cat. No. 345529)
AB_3097226 (BioLegend Cat. No. 345530)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 25 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.

Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.11.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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