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- Regulatory Status
- RUO
- Other Names
- Immunoglobluin G, IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 479604 | 5 plates | 336€ | ||||
IgG is a monomeric immunoglobulin composed of two identical heavy chains and two identical light chains linked together by inter-chain disulfide bonds. There are four IgG subclasses (IgG1, IgG2a/IgG2c (strain specific), IgG2b, and IgG3) in mouse. They differ in the size of the hinge region and the number and arrangements of the interchain disulfide bonds linking their heavy chains. Of the 5 immunoglobulin isotypes, IgG is most abundant in mammalian serum. IgG is synthesized and secreted by plasma B cells and is involved in the secondary immune response. IgG is generally participated in response to various antigens such as DNA vaccines and protection against pathogens such as bacteria and viruses. The measurement of IgG can be a diagnostic tool for certain conditions such as autoimmune diseases, gastrointestinal conditions or immunoglobulin-deficiency disorders.
BioLegend’s ELISA MAX™ Deluxe Set Mouse Total IgG contains the components necessary for the accurate quantification of natural and recombinant mouse total IgG. The set is designed for cost-effective and accurate quantification of mouse total IgG in serum and plasma. BioLegend’s ELISA MAX™ Deluxe Sets Mouse Total IgG are sensitive, accurate, and robust.
Kit Contents
- Kit Contents
-
- Mouse Total IgG ELISA MAX™ Capture Antibody (200X)
- Mouse Total IgG ELISA MAX™ Detection Antibody (200X)
- Mouse Total IgG Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution D
- Coating Buffer B (5X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 12.7 pg/mL
- Standard Range
- 62.5 – 4000 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- B cells
- Biology Area
- Adaptive Immunity, Immunology, Western Blot Controls
- Gene ID
- NA
- UniProt
- View information about IgG on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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