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ELISA MAX™ Deluxe Set Human CCL5 (RANTES)

Pricing & Availability
Regulatory Status
RUO
Other Names
D17S136E, SCYA5, SISd, TCP228
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Product Citations
publications
CCL5_Human_ELISA_Kit_Deluxe_121313
  • CCL5_Human_ELISA_Kit_Deluxe_121313
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440804 5 plates 278€
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Description

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CCL5, also known as RANTES, is a member of the CC family of chemokines. The precursor of CCL5 contains 91 amino acids, with an N-terminal signal peptide of 23 amino acids which is cleaved to generate the 68 amino acids mature protein (7.8 kD). Human mature CCL5 shares 82% - 90% amino acid seqeuence identity with equine, porcine, bovine, mouse, rat and canine CCL5.

CCL5 is expressed by T cells and monocytes/macrophages. It plays an active role in recruiting leukocytes into inflammatory sites through receptors CCR1, CCR3, CCR4, and CCR5. CCL5 induces the proliferation and activation of certain natural killer (NK) cells. It also inhibits HIV-1 entry and infection. In addition, CCL5 plays a crucial role in the migration and metastasis of cancer cells. Increased CCL5 levels are found in melanoma, breast, cervical, prostate, gastric, and pancreatic cancers.


Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human CCL5 (RANTES) ELISA MAX™ Capture Antibody (200X)
  • Human CCL5 (RANTES) ELISA MAX™ Detection Antibody (200X)
  • Human CCL5 (RANTES) Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Kirchhammer N, et al. 2022. Sci Transl Med. 14:eabm9043. PubMed
  2. Anand A, et al. 2020. Carcinogenesis. . PubMed
  3. Chabanon RM, et al. 2019. J Clin Invest. 129:1211. PubMed
  4. Yang JH, et al. 2021. Molecules. 26:. PubMed
  5. He X, et al. 2021. Cell Rep. 36:109392. PubMed
  6. Patsouras M, et al. 2015. J Autoimmun. Available online 15 August 2015. PubMed
Sensitivity
3 pg/mL
Standard Range
7.8-500 pg/ml
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, monocytes/macrophages, mesangial cells, platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 09.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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