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LEGEND MAX™ Human FGF-basic ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Fibroblast growth factor 2 (Fgf-2), Fgfb, bFGF, FGF-basic Precoated ELISA Kit
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Product Citations
publications
FGF-basic_Human_LegendMax_ELISA_062514
  • FGF-basic_Human_LegendMax_ELISA_062514
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434309 1 Pre-coated Plate 328€
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Description

Fibroblast growth factor-basic (FGF-basic) is a heparin-binding growth factor which belongs to a family of fibroblast growth factors (FGFs). FGF family members share 35-60% amino acid sequence homology and are highly conserved among species. FGF-basic has been isolated from neural, pituitary, adrenal cortical, and placental tissues. Human FGF-basic is a 17.2 kD protein containing 154 amino acids. Other isofoms (22, 22.5, 24 and 34 kD) have also been reported with distinct intracellular localizations and functions. FGFs are involved in many physiological and pathological processes including embryonic development, neuronal outgrowth, angiogenesis, and malignant transformation. FGF-basic is essential for the development and maintenance of vascular integrity during embryogenesis and is also a major angiogenic factor involved in wound healing, cardiovascular disease, and tumor neovascularization.

BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human FGF-basic specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is specifically designed for the accurate quantitation of human FGF-basic from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human FGF-basic Pre-coated 96-well Strip Microplate
  • Human FGF-basic Dectection Antibody
  • Human FGF-basic Standard
  • Matrix A
  • Avidin-HRP E
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Product Citations
  1. Kriauciunaite K, et al. 2021. J Cell Physiol. 236:7642. PubMed
Sensitivity
4 pg/mL
Standard Range
12.5 - 800 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
2247 View all products for this Gene ID
UniProt
View information about FGF-basic on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 06.25.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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