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LEGEND MAX™ Human IL-17A ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-17A ELISA Kit, IL-17 ELISA Kit
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Product Citations
publications
IL-17A_Human_LegMax_110111
  • IL-17A_Human_LegMax_110111
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433917 1 Pre-coated Plate 328€
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Description

IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-a, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis.


BioLegend’s LEGEND MAX™ Human IL-17A ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a capture antibody. This kit is specifically designed for the accurate protein quantitation of human IL-17A from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IL-17A Pre-coated 96-well Strip Microplate
  • Human IL-17A Dectection Antibody
  • Human IL-17A Standard
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Application References

(PubMed link indicates BioLegend citation)
  1. Verma NK, et al. 2012. J. Biol Chem. 287:27204. PubMed.
Product Citations
  1. Izati AF, et al. 2021. Front Immunol. 12:690908. PubMed
  2. Verma N, et al. 2012. J Biol Chem. 287: 27204. PubMed
  3. Moafi M, et al. 2017. Scand J Immunol. 85:291. PubMed
  4. Sun J, et al. 2019. Exp Ther Med. 1.752083333. PubMed
  5. Schwarzkopf K, et al. 2019. Hepatol Commun. 3:392. PubMed
Sensitivity
0.8 pg/mL
Standard Range
3.9-250 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated T cell subpopulation
Biology Area
Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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