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Lysosome Probe Orange

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A_Lysosome_Probe_Orange_072823
Live cell fluorescence imaging of RAW264.7 cells starved with serum-free and glucose-free media for 4 hours to induce lysosomal activity and treated with 200 nM Bafilomycin (left) or left untreated (right). Cells were then stained with Lysosome Probe Orange (yellow) and nuclei were stained with Hoechst 33342 (blue). Images were captured using a 60X objective. Scale bar: 10 µm
  • A_Lysosome_Probe_Orange_072823
    Live cell fluorescence imaging of RAW264.7 cells starved with serum-free and glucose-free media for 4 hours to induce lysosomal activity and treated with 200 nM Bafilomycin (left) or left untreated (right). Cells were then stained with Lysosome Probe Orange (yellow) and nuclei were stained with Hoechst 33342 (blue). Images were captured using a 60X objective. Scale bar: 10 µm
  • B_Lysosome_Probe_Orange_FC_082323
    Jurkat cells were starved in serum free, glucose free media for 4h and stained with Lysosome Probe Orange (filled histogram) or left unstained (open histogram).
See Lysosome Probe Orange spectral data
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421912 1 vial 200€
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Description

Lysosomes are cellular organelles involved in degrading cellular debris during autophagy, as well endocytosed material. Defects in lysosomal function are related to neurodegenerative disorders, cancers, and aging-related diseases. Lysosome Probe Orange labels lysosomes in live cells and selectively accumulates in lysosomes via the lysosomal pH gradient. Fluorescence is greatly enhanced upon entry into lysosomes. Its photostability and excellent cellular retention makes this probe particularly useful for live cell imaging applications, as well as for flow cytometry. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Formulation
1 vial of 100 µL 500X Lysosomal Probe in DMSO
Preparation
Please refer to detailed protocol in application notes.
Storage & Handling
-20°C
Application

Live cell imaging - Quality tested
FC - Verified
Recommended Usage

Please refer to detailed protocol in application notes.

Application Notes

Component:
20 μL of 500X Lysosome Probe Orange (-20°C, protected from light, avoid freeze/thaw cycles)

Required Materials Not Included:
Buffer: PBS, phenol-free media, or HBSS

Detection/Imaging Guidelines:
Ex/Em = 542/556 nm 
Fluorescence microscope filter set: Cy3
Flow cytometry channel: PE

Assay Protocol:

  1. Thaw the 500X Lysosome Probe and allow it to reach room temperature.
  2. Prepare lysosome staining solution by diluting the Lysosome Probe 1:500 in your buffer of choice.
    Note: Probe concentration may need to be adjusted according to cell type and assay conditions.
  3. Optional: Include live-cell nuclear counterstain such as DRAQ5™ (Cat. No. 424101).
  4. Add an equal volume of Lysosome Staining solution to cell culture media.
  5. Incubate the cells at 37°C for 30 minutes.
  6. Wash cells by twice with PBS or HBSS. If cells are in suspension, spin down and resuspend in buffer of choice.
  7. Add desired volume of PBS or HBSS to cells for observation.
  8. Proceed immediately to analysis.

Antigen Details

Distribution

Lysosomes

Function
Lysosomal activity
Biology Area
Cell Biology, Cell Structure, Protein Trafficking and Clearance
Molecular Family
Lysosomal Markers
Gene ID
NA
Go To Top Version: 2    Revision Date: 07.28.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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