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Pacific Blue™ anti-mouse I-Ab Antibody

Pricing & Availability
Clone
AF6-120.1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (BALB/c) IgG2a, κ
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Product Citations
publications
AF6-120_PB_060710
C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™
  • AF6-120_PB_060710
    C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
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116421 25 µg 85€
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116422 100 µg 207€
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Description

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10J splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Does not react with other haplotypes (e.g., d, f, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
  3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
  4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
  5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
  6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
  7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
  8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
  9. Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
Product Citations
  1. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  2. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  3. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  4. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  5. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  6. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  7. Tay S, et al. 2014. J Immunol. 193:2087. PubMed
  8. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  9. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  10. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  11. Ruscher R, et al. 2018. Bio Protoc. 8:e2757. PubMed
  12. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  13. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
RRID
AB_10613291 (BioLegend Cat. No. 116421)
AB_10613291 (BioLegend Cat. No. 116422)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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