PE anti-Bcl-2 Antibody anti-Bcl-2

Pricing & Availability

Clone: 100 (See other available formats)
Regulatory Status: RUO
Other Names: B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
Isotype: Mouse IgG1
Ave. Rating: Submit a Review
Product Citations: publications

100_PE_Bcl-2_Antibody_FC_032014 — Human peripheral blood lymphocytes were treated with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), then stained with Bcl-2 (clone 100) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

Compare all formats See PE spectral data

Cat #

Size

Price

Quantity Check Availability

Save

658707

25 tests

132€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

658708

100 tests

308€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2563281 (BioLegend Cat. No. 658707)
AB_2563281 (BioLegend Cat. No. 658708)

Antigen Details

Structure: Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
Distribution: Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
Function: Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction: BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References: 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.
Gene ID: 596 View all products for this Gene ID
UniProt: View information about Bcl-2 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Bcl-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Bcl-2	100	WB,IHC-F,IHC-P
Alexa Fluor® 647 anti-Bcl-2	100	ICFC,SB
Alexa Fluor® 488 anti-Bcl-2	100	ICFC
PE anti-Bcl-2	100	ICFC
Brilliant Violet 421™ anti-Bcl-2	100	ICFC
TotalSeq™-Bn1180 anti-Bcl-2	100	SB
TotalSeq™-B1180 anti-Bcl-2	100	ICPG

Certificate of Analysis

Customers Also Purchased

Pacific Blue™ Annexin V

PE anti-human CD127 (IL-7Rα)

Clone: A019D5

Alexa Fluor® 488 anti-Bax

Clone: 2D2

APC anti-human CD39

Clone: A1

Go To Top Version: 1 Revision Date: 03.26.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.