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PE anti-GATA2 Antibody

Pricing & Availability
Clone
W17242A (See other available formats)
Regulatory Status
RUO
Other Names
GATA Binding Protein 2, IMD21, NFE1B, DCML
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17242A_PE_GATA2_Antibody_1_043020
C57BL/6 mouse bone marrow hematopoietic stem/progenitor cells were isolated using the MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit. Isolated cells were then surface stained with CD117 APC before being treated with True-Nuclear™ Transcription Factor Buffer Set. Treated cells were then intracellularly stained with GATA2 (clone W17242A) PE (left) or rat IgG2b, κ PE isotype control (right).
  • W17242A_PE_GATA2_Antibody_1_043020
    C57BL/6 mouse bone marrow hematopoietic stem/progenitor cells were isolated using the MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit. Isolated cells were then surface stained with CD117 APC before being treated with True-Nuclear™ Transcription Factor Buffer Set. Treated cells were then intracellularly stained with GATA2 (clone W17242A) PE (left) or rat IgG2b, κ PE isotype control (right).
  • W17242A_PE_GATA2_Antibody_2_043020
    Molt-4 cells were treated with True-Nuclear™ Transcription Factor Buffer Set, then intracellularly stained with GATA2 (clone W17242A) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • W17242A_PE_GATA2_Antibody_3_043020
    HEL cells were treated with True-Nuclear™ Transcription Factor Buffer Set, then intracellularly stained with GATA2 (clone W17242A) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram)
See PE spectral data
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614003 25 µg 142€
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614004 100 µg 370€
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Description

GATA2 is a member of the GATA transription factor family. Mutations in GATA2 lead to impaired development and differentiation of hematopoietic  lineages, including dendritic cells, monocytes, B cells, and natural killer cells. Over the course of differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exhanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant partial human GATA2 protein corresponding to amino acids 1-250
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2861048 (BioLegend Cat. No. 614003)
AB_2861048 (BioLegend Cat. No. 614004)

Antigen Details

Structure
GATA2 is a 480 amino acid protein with a predicted molecular weight of 51 kD.
Distribution

Widely expressed/nucleus

Function
Transcriptional activator/Regulates developmental processes in hematopoietic cells
Cell Type
Lymphocytes
Biology Area
Cell Biology, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Orkin SH. 1992. Blood. 80: 575.
  2. Bresnick EH, et al. 2010. J. Biol. Chem. 285: 31087.
  3. Collin M, et al. 2011. Nat. Rev. Immunol. 11: 575.
  4. Doré LC, et al. 2012. Blood. 119: 3724.
Gene ID
2624 View all products for this Gene ID
UniProt
View information about GATA2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04.29.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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