- Clone
- SN2 (N6-D11) (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI N-L099
- Other Names
- SN2, gp37, AD2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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329010 | 100 tests | 215€ |
CD165 is a 37-42 kD transmembrane glycoprotein, also known as AD2, gp37, and SN2. It is expressed on immature thymocytes, most platelets, thymic epithelial cells, fibroblasts, and acute T-lymphoblastic leukemia (T-ALL) cells. CD165 has also been found to be expressed on monocytes and circulating T cells. In addition, CD165 has been detected on islet cells of the pancreas, Bowman's capsule of the kidney and on central nervous system neurons. SN2 antigen has been reported to be identical, or very similar, to the T-cell acute lymphoblastic leukemia antigen, TALLA-1. The biological function of CD165 is still not clear, though it has been proposed that CD165 plays a role in cell adhesion between thymocytes and thymic epithelial cells during development.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- purified human CD165 antigen from Molt-4 cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen tissue sections.1, Western Blotting1, and immunofluorescence microscopy1
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Application References
(PubMed link indicates BioLegend citation) -
- Seon BK, et al. 1984. J. Immunol. 132:2089.
- RRID
-
AB_2072596 (BioLegend Cat. No. 329010)
Antigen Details
- Distribution
-
immature thymocytes, acute T-lymphoblastic leukemia (T-ALL) cells, epithelial cells, fibroblasts, platelets, monocytes and circulating T cells
- Cell Type
- Epithelial cells, Fibroblasts, Leukemia, Monocytes, Platelets, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kishimoto T, et al. 1997. ed. Leucocyte Typing VI:White Cell Differentiation Antigens. London:Garland Publishing.
2. Seon BK. 1995. Int. J. Cancer. 63:474.
3. Takagi S, et al. 1995. Int. J. Cancer. 61:706. - Gene ID
- NA
- UniProt
- View information about CD165 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD165 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD165 | SN2 (N6-D11) | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD165
Human T-lymphoblastic leukemia cell line Jurkat stained with...
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