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PE anti-human CD286 (TLR6) Antibody

Pricing & Availability
Clone
TLR6.127 (See other available formats)
Regulatory Status
RUO
Other Names
TLR6, CD286
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TLR6dot127_PE_121508.jpg
Human peripheral blood monocytes stained with TLR6.127 PE
  • TLR6dot127_PE_121508.jpg
    Human peripheral blood monocytes stained with TLR6.127 PE
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334707 25 tests 137€
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334708 100 tests 247€
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Description

Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been identified. Toll-like receptor 6 (TLR6), known as CD286, is one of the important receptors. TLR6 is colocalized with TLR2 on the cell surface of monocytes/macrophages, dendritic cells, granulocytes. The TLR2/TLR6 complex plays a role in the innate immune response to diacylated lipoprotein and peptidoglycan antigens.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR6 transfected Ba/F3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: blocks cytokine production induced by MALP-2 and peptidoglycan(1)

Application References

(PubMed link indicates BioLegend citation)
  1. Nakao Y, et al. 2005. J. Immunol. 174:1566
Product Citations
  1. Whitmore L, et al. 2016. J Immunol. 196: 1376 - 1386. PubMed
RRID
AB_2205397 (BioLegend Cat. No. 334707)
AB_2205397 (BioLegend Cat. No. 334708)

Antigen Details

Structure
Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, colocalized with TLR2 at the cell surface
Distribution
Monocytes/macrophages, dendritic cells, granulocytes
Function
Activates innate host defense against diverse set of pathogens
Ligand/Receptor
Diacylated lipoprotein, peptidoglycan
Cell Type
Monocytes, Macrophages, Dendritic cells, Granulocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Takeuchi O, et al. 2001. Int. Immunol. 13:933
2. Shimizu T, et al. 2005. J. Immunol. 175:4641
3. Into T, et al. 2004. Cell Microbiol. 6:187

Gene ID
10333 View all products for this Gene ID
UniProt
View information about CD286 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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