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PE anti-human CD97 Antibody

Pricing & Availability
Clone
VIM3b (See other available formats)
Regulatory Status
RUO
Workshop
V BP030
Other Names
BL-KDD/F12
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
VIM3b_PE_100908.jpg
Human peripheral blood granulocytes stained with VIM3b PE
  • VIM3b_PE_100908.jpg
    Human peripheral blood granulocytes stained with VIM3b PE
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336307 25 tests 94€
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336308 100 tests 225€
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Description

CD97 is a seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family with molecular weights of 74 kD, 80 kD, and 86 kD. It is expressed on monocytes/macrophages, granulocytes, dendritic cells, and smooth muscle cells.The expression of CD97 on resting T- and B-lymphocytes is at low level, but is rapidly upregulated upon activation. CD97 binds to CD55 (decay-accelerating factor), chondroitin sulfate, integrin a5b1, and a3b1. CD97 has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG1a (human myeloid cell line)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone VIM3b bindswithin the first EGF domain of CD97.4 Additional reported applications include: western blot, immunoprecipitation. 

Application References

(PubMed link indicates BioLegend citation)
  1. Hamann J, et al. 1995. J. Immunol. 155:1942.
  2. Lawrence DW, et al. 2003. J. Exp. Med. 198:999.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Wobus M, et al. 2004. Int. J. Cancer 112:815.
Product Citations
  1. Nakajima H, et al. 2007. J Neurochem. 2428:103. PubMed
  2. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  3. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
RRID
AB_2076182 (BioLegend Cat. No. 336307)
AB_2076182 (BioLegend Cat. No. 336308)

Antigen Details

Structure
Seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family, 74 kD, 80 kD, 86 kD
Distribution

Monocytes/macrophages, granulocytes, dendritic cells, low levels on T cells and B-lymphocytes and upregulated upon activation

Function
Co-stimulation, adhesion
Ligand/Receptor
CD55, chondroitin sulfate, integrin α5β1, integrin α3β1
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Capasso M, et al. 2006. J. Immunol. 177:1070
2. Wang T, et al. 2005. Blood 105:2836
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication

Gene ID
976 View all products for this Gene ID
UniProt
View information about CD97 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10.23.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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