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- Clone
- DH8.3.Rec (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EGFRv3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human glioblastoma cell line, DKMG/EGFRvIII, was stained with anti-human EGFRvIII recombinant (clone DH8.3.Rec) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 335613 | 25 tests | 123€ | ||||
| 335614 | 100 tests | 273€ | ||||
Epidermal growth factor receptor variant III (EGFRvIII) arises from an in-frame deletion of exons 2 to 7 in the extracellular region of EGFR, resulting in a truncated receptor that is constitutively active. This mutation is cancer-specific and is present in approximately 50% of glioblastoma tumors.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Peptide LEEKKGNYVVTDHC conjugated to KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Antigen Details
- Structure
- Deletion of exons 2-7 of the EGFR gene. Molecular mass of approximately 145 kD.
- Distribution
-
Present in approximately 50% of glioblastoma tumors
- Function
- Pro-tumorigenic (promotes tumor growth, survival, invasion, stemness, metabolism, and angiogenesis).
- Biology Area
- Cancer Biomarkers
- Antigen References
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- Hui G, et al. 2013. FEBS Journal. 280:21.
- Gene ID
- 1956 View all products for this Gene ID
- UniProt
- View information about EGFRvIII on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All EGFRvIII Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human EGFRvIII Recombinant Antibody | DH8.3.Rec | FC |
| PE anti-human EGFRvIII Recombinant Antibody | DH8.3.Rec | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human EGFRvIII Recombinant Antibody
Human glioblastoma cell line, DKMG/EGFRvIII, was stained wit... -
PE anti-human EGFRvIII Recombinant Antibody
Human glioblastoma cell line, DKMG/EGFRvIII, was stained wit...
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