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PE anti-human IL-4

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
MP4-25D2
Other Names
Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Rat IgG1, κ
MP4-25D2_PE_IL-4_ASR_022823
Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with MP4-25D2 PE used at 5 µL/test (left) or with an isotype control (right).
  • MP4-25D2_PE_IL-4_ASR_022823
    Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with MP4-25D2 PE used at 5 µL/test (left) or with an isotype control (right).
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986602 500 µL 258€
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Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
25 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Intracellular Staining for Flow Cytometry
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
  2. Boulay J and Paul WE. 1992. Curr Opin Immunol. 4:294-8.
  3. Dullens H and De Wit CL. 1991. In Vivo. 5:567-70.
  4. Paul W. 1991. Blood. 77:1859-70.
Go To Top Version: 1    Revision Date: 03.06.2023

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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