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PE anti-human LAP (TGF-β1) Antibody

Pricing & Availability
Clone
S20006A (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S20006A_PE_LAP_TGFbeta1_Antibody_081021.png
Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with GARP (LRRC32) PerCP/Cyanine5.5 and anti-human LAP (TGFβ1 Clone S20006A) PE (left) or mouse IgG1, κ PE isotype control (right).
  • S20006A_PE_LAP_TGFbeta1_Antibody_081021.png
    Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with GARP (LRRC32) PerCP/Cyanine5.5 and anti-human LAP (TGFβ1 Clone S20006A) PE (left) or mouse IgG1, κ PE isotype control (right).
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300003 25 tests 113€
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300004 100 tests 283€
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Description

TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TGF-β1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S2006A can completely block the binding of clones TW4-2F8 and TW4-6H10 on target cells.

Product Citations
  1. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
RRID
AB_2894444 (BioLegend Cat. No. 300003)
AB_2894444 (BioLegend Cat. No. 300004)

Antigen Details

Structure
About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
Distribution

Ubiquitously expressed by many kinds of cells

Function
Multifunctional cytokine, regulates cells proliferation, and differentiation
Ligand/Receptor
TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
Cell Type
B cells, Dendritic cells, T cells, Tregs
Biology Area
Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Inhibitory Molecules, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Yi JJ, et al. 2010. Cell. 142:144.
  2. Tran DQ, et al. 2009. Blood. 113:5125.
  3. Lu M, et al. 2002. J Cell Sci. 115:4641.
  4. Khalil N. 1999. Microbes Infect. 1:1255.
Gene ID
7040 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08.10.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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