PE anti-human Pre-BCR Antibody

Pricing & Availability
Clone
HSL2 (See other available formats)
Regulatory Status
RUO
Other Names
Pre-B cell Receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HSL2_PE_031110
Human pre-B cell line Nalm-6 intracellularly stained with HSL2 PE
  • HSL2_PE_031110
    Human pre-B cell line Nalm-6 intracellularly stained with HSL2 PE
See PE spectral data
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347904 100 tests 317€
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Description

The human Pre-BCR is composed of mH chain and CD179a (VpreB)/CD179b (λ5). The mAb HSL2 reacts with a conformational epitope unique to the Pre-BCR complex. The Pre-BCR associates with the signal transducing heterodimer, CD79a/CD79b, to form the Pre-BCR complex. Pre-BCR is expressed on Pro-B and early Pre-B cells and plays a critical role in B cell development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tsuganezawa K, et al. 1998. Blood 92:4317.
  2. Kiyokawa N, et al. 2004. Modern Pathology 17:423.
RRID
AB_2063902 (BioLegend Cat. No. 347904)

Antigen Details

Structure
Ig superfamily, Pre-BCR is compose of CD179a, CD179b andµH
Distribution

Pro-B and early Pre-B cells

Cell Type
B cells
Biology Area
Immunology
Antigen References

1. LeBien TW, et al. 2000. Blood 96:9.
2. Zhang M, et al. 2004. Cell Mol Immunol. 1:89.

Gene ID
613 View all products for this Gene ID
UniProt
View information about Pre-BCR on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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