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PE anti-mouse CD24 Antibody

Pricing & Availability
Clone
M1/69 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Isotype
Rat IgG2b, κ
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Product Citations
publications
M1slash69_PE_072808.jpg
C57BL/6 mouse splenocytes stained with M1/69 PE
  • M1slash69_PE_072808.jpg
    C57BL/6 mouse splenocytes stained with M1/69 PE
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101807 50 µg 69€
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101808 200 µg 196€
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Description

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Howley BV, et al. 2018. Oncogene. 37:1308. PubMed
  2. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  3. Schmidt V, et al. 2021. J Cell Biol. 220:. PubMed
  4. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  5. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  6. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  7. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  8. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  9. Lichtenberger B, Watt M 2016. Nat Commun. 7:10537. PubMed
  10. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  11. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  12. Sakaguchi S, et al. 2015. J Leukoc Biol. 97:635. PubMed
  13. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  14. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  15. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  16. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  17. Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed
  18. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  19. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  20. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  21. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  22. Gu VW, et al. 2021. Stem Cell Reports. 16:106. PubMed
  23. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  24. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  25. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  26. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  27. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  28. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
  29. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  30. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  31. Chen C, et al. 2008. Endocrinology. 150:492. PubMed
RRID
AB_312840 (BioLegend Cat. No. 101807)
AB_312840 (BioLegend Cat. No. 101808)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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