- Clone
- 7H11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNGR-1, C-type lectin domain family 9 member A, CD370
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
143503 | 25 µg | 94€ | ||||
143504 | 100 µg | 268€ |
CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM). In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB)
- Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)
- Product Citations
-
- RRID
-
AB_11147366 (BioLegend Cat. No. 143503)
AB_11147366 (BioLegend Cat. No. 143504)
Antigen Details
- Structure
- Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
- Distribution
-
Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells
- Function
- Cross present antigen to CTL
- Interaction
- Necrotic cells
- Ligand/Receptor
- Ubiquitous preformed acid-labile protein associated ligand(s)
- Cell Type
- Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens
- Antigen References
-
Poulin LF, et al. 2012. Blood 119:6052.
- Gene ID
- 232414 View all products for this Gene ID
- UniProt
- View information about CD370 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD370 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD370 (CLEC9A, DNGR1) | 7H11 | FC,ELISA,ICC,IP,WB |
PE anti-mouse CD370 (CLEC9A, DNGR1) | 7H11 | FC |
APC anti-mouse CD370 (CLEC9A, DNGR1) | 7H11 | FC |
TotalSeq™-C0556 anti-mouse CD370 (CLEC9A, DNGR1) | 7H11 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD370 (CLEC9A, DNGR1)
-
PE anti-mouse CD370 (CLEC9A, DNGR1)
C57BL/6 mouse splenocytes were stained with CD11c APC, CD8a ... -
APC anti-mouse CD370 (CLEC9A, DNGR1)
C57BL/6 mouse splenocytes were stained with CD11c PE, CD8&al... -
TotalSeq™-C0556 anti-mouse CD370 (CLEC9A, DNGR1)
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