Loading...

PE anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
X54-57.1_1_PE_062410
C57BL/6 bone marrow cells stained with CD11b FITC and X54-5/7.1 PE
  • X54-57.1_1_PE_062410
    C57BL/6 bone marrow cells stained with CD11b FITC and X54-5/7.1 PE
  • X54-57.1_2_PE_062410
    C57BL/6 bone marrow cells stained with CD11b FITC and mouse IgG1 PE isotype control
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
139303 25 µg 94€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
139304 100 µg 237€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  2. Afkhami S, et al. 2022. Cell. 185:896. PubMed
  3. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  4. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  5. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  6. West HC, et al. 2022. Cell Rep. 39:110819. PubMed
  7. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  8. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  9. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  10. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  11. Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
  12. Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed
  13. Zhong WJ, et al. 2023. J Transl Med. 21:179. PubMed
  14. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  15. Song HY, et al. 2023. Front Immunol. 14:1182927. PubMed
  16. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  17. Israelow B, et al. 2020. bioRxiv. . PubMed
  18. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  19. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  20. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
  21. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  22. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  23. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  24. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  25. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  26. Mansouri N, et al. 2019. JCI Insight. 4:e128060. PubMed
  27. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  28. Langlet C, et al. 2012. J Immunol. 188:1751. PubMed
  29. Okamoto F, et al. 2010. J Biol Chem. 285:41113. PubMed
  30. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  31. Pott J et al. 2018. Cell host & microbe. 23(2):191-202 . PubMed
  32. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  33. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  34. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  35. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  36. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  37. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  38. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  39. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  40. Karsten C, et al. 2015. J Immunol. 194:1841. PubMed
  41. Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
  42. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  43. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  44. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  45. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  46. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  47. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  48. Man F, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L803. PubMed
  49. Zhang X, et al. 2020. Elife. 9:00. PubMed
  50. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  51. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  52. Tkacz K, et al. 2020. Cells. 9:. PubMed
  53. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  54. Zhang F, et al. 2020. EMBO Mol Med. e12034:12. PubMed
  55. D'Agostino MR, et al. 2022. STAR Protoc. 3:101652. PubMed
  56. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  57. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  58. Cnops J, et al. 2015. PLoS Pathog. 11:1004964. PubMed
  59. Hung W, et al. 2016. Sci Rep. 6:35343. PubMed
  60. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  61. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  62. Jacobson A et al. 2018. Cell host & microbe. 24(2):296-307 . PubMed
  63. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  64. Cheresh P, et al. 2020. Int J Mol Sci. 21:. PubMed
  65. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  66. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  67. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  68. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  69. Yao Y, et al. 2018. Cell. 175:1634. PubMed
  70. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  71. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  72. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  73. Jiang Q, et al. 2020. JCI Insight. 5:00. PubMed
  74. Wang Y et al. 2017. Cell. 171(2):331-345 . PubMed
  75. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  76. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  77. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  78. Ferrer-Font L, et al. 2020. Elife. 9:. PubMed
  79. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  80. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  81. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  82. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  83. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  84. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  85. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  86. Huangfu LX, et al. 2021. Exp Ther Med. 22:1433. PubMed
  87. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  88. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  89. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  90. Smith LK, et al. 2021. Elife. 10:. PubMed
  91. Droho S, et al. 2020. J Vis Exp. . PubMed
  92. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  93. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  94. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  95. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  96. Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
  97. Cho K, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L429. PubMed
  98. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  99. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  100. Stijlemans B, et al. 2015. PLoS Negl Trop Dis. 9:3561. PubMed
RRID
AB_10613467 (BioLegend Cat. No. 139303)
AB_10613467 (BioLegend Cat. No. 139304)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12.14.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account