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PE anti-mouse IgMa Antibody

Pricing & Availability
Clone
MA-69 (See other available formats)
Regulatory Status
RUO
Other Names
Igh-6a
Isotype
Mouse (C57BL/6) IgG1, κ
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Product Citations
publications
MA-69_PE_052609.jpg
Balb/c mouse splenocytes stained with MA-69 PE
  • MA-69_PE_052609.jpg
    Balb/c mouse splenocytes stained with MA-69 PE
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Cat # Size Price Quantity Check Availability Save
408608 200 µg 283€
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Description

This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Sieckmann D, et al. 1991. Hybridoma 10:121.
  2. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  3. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  4. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  5. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  6. Smith LK, et al. 2021. Elife. 10:. PubMed
  7. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID
AB_940545 (BioLegend Cat. No. 408608)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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