Loading...

PE anti-mouse/rat/human FOXP3 Antibody

Pricing & Availability
Clone
150D (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
150D_PE_FOXP3_Antibody_ICFC_1_041515
C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 150D_PE_FOXP3_Antibody_ICFC_1_041515
    C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone 150D) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 150D_PE_FOXP3_Antibody_ICFC_2_041515
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
320007 25 tests 162€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
320008 100 tests 317€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Cynomolgus, Rhesus, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
  2. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol
  3. Yang ZZ, et al. 2006. Blood 107:3639.
  4. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659.
  5. Groh V, et al. 2006. Nature Immunology 7:755.
  6. Lewkowicz P, et al. 2006 J. Immunol. 177:7155.
  7. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023.
  8. Bamias G, et al. 2007. J. Immunol. 178:1809.
  9. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed
  10. Davidson TS, et al. 2007. J. Immunol. 178:4022.
  11. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.
  12. Jaffar Z, et al. 2007. J. Immunol. 179:6193.
  13. Müller M, et al. 2007. J. Immunol. 179:2774.
  14. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed
  15. Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed
  16. Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed
  17. Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381
  18. Klunker S, et al. 2009. J. Exp Med. PubMed
  19. Haque A, et al. 2010. J. Immunol. 184:2583. PubMed
  20. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
Product Citations
  1. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  2. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  3. Barsheshet Y, et al. 2022. Int J Mol Sci. 23:. PubMed
  4. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  5. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  6. Wu X, et al. 2023. iScience. 26:106559. PubMed
  7. Chamorro BM, et al. 2023. Vaccines (Basel). 11:. PubMed
  8. Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed
  9. Mishra R, et al. 2022. J Transl Med. 20:323. PubMed
  10. Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  11. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  12. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  13. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  14. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  15. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  16. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  17. Zongyi Y, et al. 2017. PLoS One. 10.1371/journal.pone.0189617. PubMed
  18. Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed
  19. Levi J, et al. 2020. J Nucl Med. . PubMed
  20. Deng Z, et al. 2020. World J Gastrointest Oncol. 1091:12. PubMed
  21. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  22. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  23. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  24. Tian X, et al. 2022. J Cell Mol Med. 26:693. PubMed
  25. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  26. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  27. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  28. Yang Z, et al. 2021. Onco Targets Ther. 14:4239. PubMed
  29. Fang J, et al. 2014. J Neuroimmunol. 269:9. PubMed
  30. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  31. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  32. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  33. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  34. He S, et al. 2021. Front Pharmacol. 12:743837. PubMed
  35. Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed
  36. Tanaka S, et al. 2016. Appl Envir Microbiol. 82: 2693 - 2699. PubMed
  37. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  38. Jia Y, et al. 2022. J Transl Med. 20:281. PubMed
  39. Chen KH, et al. 2019. J Neuropathol Exp Neurol. 78:65. PubMed
  40. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  41. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  42. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  43. Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
RRID
AB_492980 (BioLegend Cat. No. 320007)
AB_492980 (BioLegend Cat. No. 320008)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
20371 View all products for this Gene ID 317382 View all products for this Gene ID 50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 3    Revision Date: 04.15.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account