PE anti-NF-κB p65 Antibody

Pricing & Availability
Clone
14G10A21 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
14G10A23_PE_NF-kBp65_Antibody_FC_111213.jpg
Human lung adenocarcinoma epithelial cell line A549 was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with NF-κB p65 (clone 14G10A21) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 14G10A23_PE_NF-kBp65_Antibody_FC_111213.jpg
    Human lung adenocarcinoma epithelial cell line A549 was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with NF-κB p65 (clone 14G10A21) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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653003 25 tests 123€
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653004 100 tests 288€
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Description

NF-κB p65 is a member of REL-like domain containing protein family, which forms a NF-κB complex with the other family members: NF-κB1 (p105/p50) or NF-κB2 (p100/p52). The NF-κB complex is inactivated and held in the cytoplasm by the NF-κB inhibitor IκB. In response to activation stimuli, IκB kinases (IKKs) phosphorylates IκB, resulting in degradation of IκB and liberation of NF-κB complex. The activated NF-κB complex translocates to the nucleus and binds to κB sites in the DNA of their target genes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NF-κB p65 recombinant protein (451-551 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2562768 (BioLegend Cat. No. 653003)
AB_2562768 (BioLegend Cat. No. 653004)

Antigen Details

Structure
65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.
Distribution

The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.

Function
NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.
Interaction
Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184.
2. Saccani S, et al. 2004. J. Exp. Med. 200:107.
3. Nolan GP, et al. 1991. Cell 64:961.
4. Chen LF, et al. 2001. Science 293:1653.
5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593.
6. Chapman NR, et al. 2002. Biochem J. 366:459.

Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p65 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.19.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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