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PE anti-XBP-1s Antibody

Pricing & Availability
Clone
143F (See other available formats)
Regulatory Status
RUO
Other Names
X-box binding protein 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
143F_PE_Xbp-1s_Antibody_FC_1_061714
Human hepatocellular carcinoma cell line, HEPG2, was incubated overnight with (top) or without (bottom) thapsigargin (300 nM). Cells were then treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-Human Xbp-1s (clone 143F) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 143F_PE_Xbp-1s_Antibody_FC_1_061714
    Human hepatocellular carcinoma cell line, HEPG2, was incubated overnight with (top) or without (bottom) thapsigargin (300 nM). Cells were then treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-Human Xbp-1s (clone 143F) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 143F_PE_Xbp-1s_Antibody_FC_2_061714
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647503 25 tests 123€
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647504 100 tests 298€
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Description

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
XBP-1s recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
  2. Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
  3. Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
  4. Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
  5. Wang L, et al. 2011. Hum Mol Genet. PubMed
  6. Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
  7. Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
  8. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  9. Shirley CM, et al. 2011 Blood. 117:6297. PubMed
  10. Vidal RL., et al. 2012. Hum Mol Genet. PubMed
  11. Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
  12. Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
  13. Bonetti P, et al. 2013. Blood. 122:2233. PubMed
  14. Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
  15. Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations
  1. Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed
RRID
AB_2563447 (BioLegend Cat. No. 647503)
AB_2563447 (BioLegend Cat. No. 647504)

Antigen Details

Distribution

Ubiquitously expressed.

Function
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area
Cell Biology
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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