PE/Cyanine5 anti-human CD62P P-Selectin Antibody anti-CD62P

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Clone: AK4 (See other available formats)
Regulatory Status: RUO
Workshop: VI P-44
Other Names: GMP-140, PADGEM
Isotype: Mouse IgG1, κ
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Product Citations: publications

AK4_PECy5_070507 — Thrombin-activated human peripheral blood platelets stained with AK4 PE/Cyanine5

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304908

100 tests

238€

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Description

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁴ and in vitro blocking of adhesion of platelets¹. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
RRID: AB_314480 (BioLegend Cat. No. 304908)

Antigen Details

Structure: Selectin, type I glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.
Gene ID: 6403 View all products for this Gene ID
UniProt: View information about CD62P on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Agranulocyte Adhesion and Diapedesis

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Other Formats

View All CD62P Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD62P (P-Selectin)	AK4	FC
FITC anti-human CD62P (P-Selectin)	AK4	FC
PE anti-human CD62P (P-Selectin)	AK4	FC
PE/Cyanine5 anti-human CD62P (P-Selectin)	AK4	FC
Purified anti-human CD62P (P-Selectin)	AK4	FC,IHC-F
Biotin anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 488 anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 647 anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 605™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 421™ anti-human CD62P (P-Selectin)	AK4	FC
PE/Cyanine7 anti-human CD62P (P-Selectin)	AK4	FC
PerCP/Cyanine5.5 anti-human CD62P (P-Selectin)	AK4	FC
Alexa Fluor® 700 anti-human CD62P (P-Selectin)	AK4	FC
PE/Dazzle™ 594 anti-human CD62P (P-Selectin)	AK4	FC
APC/Fire™ 750 anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-A0218 anti-human CD62P (P-Selectin)	AK4	PG
Brilliant Violet 650™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 510™ anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 711™ anti-human CD62P (P-Selectin)	AK4	FC
APC/Cyanine7 anti-human CD62P (P-Selectin)	AK4	FC
Brilliant Violet 785™ anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-C0218 anti-human CD62P (P-Selectin)	AK4	PG
Ultra-LEAF™ Purified anti-human CD62P (P-Selectin)	AK4	FC
TotalSeq™-B0218 anti-human CD62P (P-Selectin) Antibody	AK4	PG
TotalSeq™-D0218 anti-human CD62P (P-Selectin)	AK4	PG
PE anti-human CD62P	AK4	FC
FITC anti-human CD62P	AK4	FC
GMP FITC anti-human CD62P (P-Selectin)	AK4	FC
GMP PE anti-human CD62P (P-Selectin)	AK4	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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