PE/Cyanine5 anti-mouse CD137 Antibody, CD137, 17B5

Pricing & Availability

Clone: 17B5 (See other available formats)
Regulatory Status: RUO
Other Names: 4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CD137
Isotype: Syrian Hamster IgG
Ave. Rating: Submit a Review
Product Citations: publications

17B5_PE-CYA5_CD137_FC_100124-2 — Con A + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with anti-mouse CD3 (clone 17A2) APC and anti-mouse CD137 (clone 17B5) PE/Cyanine5 (left) or Syrian Hamster IgG PE/Cyanine5 isotype control (right).

Compare all formats See PE/Cyanine5 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

106123

25 µg

141€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

106124

100 µg

306€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

CD137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CD137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CD137 with its ligand, 4-1BBL (CD137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation². The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106108).

Antigen Details

Structure: TNF-R superfamily, 39 kD
Distribution: Activated T cells and B cells
Function: T-B cell costimulation
Ligand/Receptor: 4-1BB ligand (CDw137L)
Cell Type: B cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 21942 View all products for this Gene ID
UniProt: View information about CD137 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

TNF Superfamily Pathway

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD137 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD137	17B5	FC
PE anti-mouse CD137	17B5	FC
APC anti-mouse CD137	17B5	FC
TotalSeq™-A0194 anti-mouse CD137	17B5	PG
Ultra-LEAF™ Purified anti-mouse CD137	17B5	FC,Block
TotalSeq™-C0194 anti-mouse CD137	17B5	PG
TotalSeq™-B0194 anti-mouse CD137	17B5	PG
PE/Cyanine5 anti-mouse CD137	17B5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.