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PE/Cyanine7 anti-β Catenin 1 Antibody

Pricing & Availability
Clone
15B8 (See other available formats)
Regulatory Status
RUO
Other Names
β-catenin, CTNNB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15B8_PECyanine7_betaCatenin1_Antibody_060922
Human colon adenocarcinoma cell line SW480 were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 15B8) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine (open historgram).
  • 15B8_PECyanine7_betaCatenin1_Antibody_060922
    Human colon adenocarcinoma cell line SW480 were treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 15B8) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine (open historgram).
Compare all formats See PE/Cyanine7 spectral data
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862605 25 tests 165€
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862606 100 tests 344€
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Description

β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Guo J, et al. 2018. Cell Res. 28:1141 (IHC-P)
  2. Kisoh K, et al. 2017. Mol Neurobiol. 54:7917 (WB)
  3. Uribe RA, et al. 2015. J Cell Biol. 4:815 (IHC-F)
RRID
AB_2927830 (BioLegend Cat. No. 862605)
AB_2927830 (BioLegend Cat. No. 862606)

Antigen Details

Structure
β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular

Function
β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
Interaction
E-cadherin/CDH1, LEF1, TCF4, etc.
Antigen References
  1. Dias C, et al. 2014. Nature. 516(7529): 51
  2. Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3
  3. Na K, et al. 2017. Anticancer Res. 37(6):3249
  4. Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
Gene ID
1499 View all products for this Gene ID 12387 View all products for this Gene ID 84353 View all products for this Gene ID
UniProt
View information about beta Catenin 1 on UniProt.org
Go To Top Version: 1    Revision Date: 06.09.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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