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PE/Cyanine7 anti-human TdT Antibody

Pricing & Availability
Clone
41A (See other available formats)
Regulatory Status
RUO
Other Names
Terminal-Deoxynucleotidyl Transferase, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal transferase, DNTT
Isotype
Mouse IgG2a, κ
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Product Citations
publications
41A_PE-CYA7_TdT_Antibody_020224
Human pre-B cell line REH was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-human TdT (clone 41A) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • 41A_PE-CYA7_TdT_Antibody_020224
    Human pre-B cell line REH was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-human TdT (clone 41A) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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368811 25 tests 165€
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368812 100 tests 344€
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Description

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human TdT
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_3097578 (BioLegend Cat. No. 368811)
AB_3097578 (BioLegend Cat. No. 368812)

Antigen Details

Distribution

pre-B and pre-T lymphocytes, malignant pre-B and pre-T lymphocytes.

Function
Catalyzes the addition of deoxynucleotides to non-germ line elements at the junctions of rearranged Ig heavy chain and T cell receptor gene segments and thus produces antigen receptor diversity.
Interaction
PCNA, LPTS-RP1, LPTS-RP2, histones H2A, H2B, H3, and H4.
Ligand/Receptor
This process doesn't need a complementary DNA template.
Cell Type
B cells, T cells
Biology Area
Cell Biology, DNA Repair/Replication, Immunology
Molecular Family
Enzymes and Regulators
Antigen References

1. Yunk L, et al. 2009. J. Immunol. 183:452.
2. Kedzierska K, et al. 2008. J. Immunol. 181:2556.
3. Fuller SA, et al. 1985. Biochem. J. 231:105.
4. Feeney AJ, et al. 2001. J. Immunol. 167:3486.
5. Komori, T, et al. 1993. Science 261:1171.

Gene ID
1791 View all products for this Gene ID
UniProt
View information about TdT on UniProt.org
Go To Top Version: 1    Revision Date: 02.05.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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