PE/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse PD-1 transfected BHK cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The CD279 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC¹.

Application References

(PubMed link indicates BioLegend citation)

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3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed

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See More

RRID

AB_572016 (BioLegend Cat. No. 109109)
AB_572016 (BioLegend Cat. No. 109110)

Antigen Details

Structure

Ig superfamily, 50-55 kD

Distribution

Subset of double negative thymocytes, activated T and B cells

Function

Lymphocyte clonal selection, peripheral tolerance

Ligand/Receptor

PD-L1 (B7-H1), PD-L2

Cell Type

B cells, T cells, Thymocytes, Tregs

Biology Area

Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules

Molecular Family

CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID

18566 View all products for this Gene ID

UniProt

View information about CD279 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis