Loading...

PE/Cyanine7 anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
XMG1-2_PECyanine7_IFN-y_Antibody_1_041124
PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with B220 (RA3-6B2) APC and XMG1.2 PE/Cyanine7
  • XMG1-2_PECyanine7_IFN-y_Antibody_1_041124
    PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with B220 (RA3-6B2) APC and XMG1.2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
505825 25 µg 106€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
505826 100 µg 243€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  4. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  5. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  6. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  7. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  8. Wanhainen KM, et al. 2022. Cancer Immunol Res. 10:871. PubMed
  9. Wu J, et al. 2023. Bioact Mater. 20:259. PubMed
  10. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  11. Ge Y, et al. 2022. iScience. 25:105437. PubMed
  12. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  13. Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed
  14. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  15. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  16. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  17. Li H, et al. 2023. Front Immunol. 13:1056447. PubMed
  18. Wu B, et al. 2023. J Immunother Cancer. 11: . PubMed
  19. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  20. Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed
  21. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  22. Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed
  23. Zander R, et al. 2022. Immunity. 55:475. PubMed
  24. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  25. He Y, et al. 2022. Adv Healthc Mater. 11:e2200905. PubMed
  26. Guo A, et al. 2022. Nature. 607:135. PubMed
  27. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  28. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  29. Qiu C, et al. 2022. Front Bioeng Biotechnol. 10:1027619. PubMed
  30. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  31. Le DT, et al. 2023. iScience. 26:106059. PubMed
  32. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  33. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  34. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  35. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  36. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  37. Nakata J, et al. 2018. Oncotarget. 9:36029. PubMed
  38. He Y, et al. 2021. Infect Immun. 89:. PubMed
  39. Geng Z, et al. 2021. Nat Commun. 12:6584. PubMed
  40. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  41. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  42. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  43. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  44. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  45. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
  46. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  47. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  48. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  49. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  50. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  51. Saito S, et al. 2020. Nutrients. 12:. PubMed
  52. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  53. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  54. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  55. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  56. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  57. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  58. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  59. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  60. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  61. Marangoni F, et al. 2021. Cell. . PubMed
  62. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  63. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  64. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  65. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  66. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  67. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  68. Glennie N, et al. 2015. J Exp Med. 212: 1405-1414. PubMed
  69. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  70. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
  71. Voisinne G, et al. 2022. Nat Immunol. 23:1355. PubMed
  72. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  73. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  74. Sawant D, et al. 2014. J Immunol. 192:2904. PubMed
  75. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  76. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  77. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  78. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  79. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  80. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  81. Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed
  82. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  83. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  84. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  85. Xu S, et al. 2021. Immunity. . PubMed
  86. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  87. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  88. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  89. Meadows D, et al. 2015. PLoS One. 10: 0143738. PubMed
  90. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  91. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  92. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  93. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  94. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  95. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  96. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  97. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  98. Wu B, et al. 2020. Int J Biol Sci. 16:1526. PubMed
  99. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  100. Saigusa R, et al. 2018. Arthritis Res Ther. 20:23. PubMed
  101. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  102. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  103. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  104. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  105. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  106. Abhyankar MM, et al. 2018. NPJ Vaccines. 3:22. PubMed
  107. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  108. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  109. Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed
  110. Li C, et al. 2018. Cell. 174:285. PubMed
  111. Resende M, et al. 2019. J Immunol. 203:2451. PubMed
  112. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  113. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  114. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  115. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  116. Sun X, et al. 2021. Nature. 599:673. PubMed
  117. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  118. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  119. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  120. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  121. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  122. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  123. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  124. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  125. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  126. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  127. Wen J, et al. 2015. J Pharmacol Sci. 128: 116-124. PubMed
  128. Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
  129. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  130. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  131. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  132. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  133. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  134. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  135. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  136. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  137. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  138. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  139. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  140. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  141. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  142. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
  143. Kalim KW, et al. 2021. Front Immunol. 12:726393. PubMed
RRID
AB_2295770 (BioLegend Cat. No. 505825)
AB_2295770 (BioLegend Cat. No. 505826)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account