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PE/Cyanine7 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
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Product Citations
publications
8.1.1_PECy7_060710
Mouse thymic epithelial stromal cell line TE-71 stained with 8.1.1 PE/Cyanine7
  • 8.1.1_PECy7_060710
    Mouse thymic epithelial stromal cell line TE-71 stained with 8.1.1 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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127411 25 µg 118€
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127412 100 µg 254€
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed
  3. Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed
  4. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  5. Lütge M, et al. 2023. Nat Immunol. . PubMed
  6. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  7. Quaife-Ryan GA, et al. 2020. Development. 147: . PubMed
  8. McCarthy N, et al. 2020. Cell Stem Cell. 391:26. PubMed
  9. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  10. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  11. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  12. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  13. Ferreirinha P, et al. 2022. Development. 149: . PubMed
  14. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  15. Auvinen K, et al. 2019. Sci Rep. 9:15698. PubMed
  16. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  17. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  18. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  19. Hong SP, et al. 2020. Nat Commun. 3.306944444. PubMed
  20. Woods S, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:912. PubMed
  21. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  22. Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
  23. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  24. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  25. Urbanska AM, et al. 2020. Cancer Biother Radiopharm. 35:459. PubMed
  26. Sitnik K, et al. 2012. J Immunol. 188:4801. PubMed
  27. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  28. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  29. Benezech C, Caamano J 2013. J Vis Exp. 82: 50952. PubMed
  30. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  31. Quaife-Ryan GA, et al. 2020. Development. . PubMed
  32. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  33. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
RRID
AB_10613294 (BioLegend Cat. No. 127411)
AB_10613294 (BioLegend Cat. No. 127412)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org
Go To Top Version: 2    Revision Date: 02.03.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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