- Clone
- M3/38 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M3slash38_PECy7_Mac-2_Galectin-3_Antibody_102617 M3slash38_PECy7_Mac-2_Galectin-3_Antibody_102617](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M3slash38_PECy7_Mac-2_Galectin-3_Antibody_102617.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with Mac-2 antibody (clone M3/38) PE/Cyanine7 (filled histogram) or rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
125417 | 25 µg | 128€ | ||||
125418 | 100 µg | 296€ |
Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Raised against galectin-3 of mouse origin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.
Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
-
Application References
(PubMed link indicates BioLegend citation) -
- Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
- Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
- Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
- Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
- Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
- Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
- Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
- Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
- Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
- Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
- Product Citations
-
- RRID
-
AB_2721687 (BioLegend Cat. No. 125417)
AB_2721688 (BioLegend Cat. No. 125418)
Antigen Details
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. - Gene ID
- 16854 View all products for this Gene ID 3958 View all products for this Gene ID
- UniProt
- View information about Mac-2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Mac-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC,ICC,WB,IP,IHC,ELISA |
Biotin anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC,IHC |
PE anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC |
Alexa Fluor® 647 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC,IHC-P |
Alexa Fluor® 488 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC |
Alexa Fluor® 594 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | IHC-F |
Brilliant Violet 421™ anti-mouse/human Mac-2 (Galectin-3) | M3/38 | IHC-F |
PE/Cyanine7 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC |
APC anti-mouse/human Mac-2 (Galectin-3) | M3/38 | FC |
TotalSeq™-A0895 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | PG |
TotalSeq™-C0895 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | PG |
TotalSeq™-B0895 anti-mouse/human Mac-2 (Galectin-3) | M3/38 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal macrophages stained with M3/38 purified, f... HeLa cells were stained with purified anti-Galectin 3 (M3/38... Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat ce... Immunoprecipitation/Western Blot analysis using anti‐mouse/h... -
Biotin anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal marcophages stained with M3/38 biotin, fol... -
PE anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal marcophages stained with M3/38 PE -
Alexa Fluor® 647 anti-mouse/human Mac-2 (Galectin-3)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... Human paraffin-embedded intestine tissue slices were prepare... -
Alexa Fluor® 488 anti-mouse/human Mac-2 (Galectin-3)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Alexa Fluor® 594 anti-mouse/human Mac-2 (Galectin-3)
C57BL/6 mouse frozen colon section was fixed with 4% parafor... -
Brilliant Violet 421™ anti-mouse/human Mac-2 (Galectin-3)
C57BL/6 mouse frozen colon section was fixed with 4% parafor... -
PE/Cyanine7 anti-mouse/human Mac-2 (Galectin-3)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
APC anti-mouse/human Mac-2 (Galectin-3)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
TotalSeq™-A0895 anti-mouse/human Mac-2 (Galectin-3)
-
TotalSeq™-C0895 anti-mouse/human Mac-2 (Galectin-3)
-
TotalSeq™-B0895 anti-mouse/human Mac-2 (Galectin-3)
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