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PE/Dazzle™ 594 anti-human IL-10 Antibody

Pricing & Availability
Clone
JES3-9D7 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
JES3-9D7_PEDAZL594_IL10_Antibody_1_012016
LPS-stimulated human peripheral blood mononuclear cells (in the presence of monensin) were surface stained with CD14 FITC, fixed, permeabilized, and then stained with IL-10 (clone JES3-9D7) PE/Dazzle™ 594 (top) or rat IgG1, κ PE/Dazzle™ 594 isotype control (bottom). Data shown was gated on monocyte population.
  • JES3-9D7_PEDAZL594_IL10_Antibody_1_012016
    LPS-stimulated human peripheral blood mononuclear cells (in the presence of monensin) were surface stained with CD14 FITC, fixed, permeabilized, and then stained with IL-10 (clone JES3-9D7) PE/Dazzle™ 594 (top) or rat IgG1, κ PE/Dazzle™ 594 isotype control (bottom). Data shown was gated on monocyte population.
  • JES3-9D7_PEDAZL594_IL10_Antibody_2_012016
Compare all formats See PE/Dazzle™ 594 spectral data
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501425 25 tests 113€
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501426 100 tests 278€
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Rhesus, Cynomolgus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS - expressed, recombinant human IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intraceullular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture1-5 or ELISPOT Capture6: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining12.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
  2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
  3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
  4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
  5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
  6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
  7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
  8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
  9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
  10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
  11. Mason GM, et al. 2012. PNAS. PubMed
  12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)
Product Citations
  1. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  2. Saggau C, et al. 2022. Immunity. :. PubMed
  3. Bacher P, et al. 2020. Immunity. . PubMed
  4. Bacher P et al. 2019. Cell. 176(6):1340-1355 . PubMed
  5. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  6. Feuerer N, et al. 2022. Biomedicines. 10:. PubMed
RRID
AB_2566744 (BioLegend Cat. No. 501425)
AB_2566744 (BioLegend Cat. No. 501426)

Antigen Details

Structure
Acid-labile cytokine, dimer, 35-40 kD (Mammalian)
Bioactivity
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Cell Sources
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation
Production inhibited by IL-4, IL-10
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 1    Revision Date: 01.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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