PE/Fire™ 744 anti-human CD69 Antibody, CD69, FN50

Pricing & Availability

Clone: FN50 (See other available formats)
Regulatory Status: RUO
Workshop: IV A91
Other Names: Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype: Mouse IgG1, κ
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Product Citations: publications

A_FN50_PEFire744_CD69_Antibody_060324 — Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD69 (clone FN50) PE/Fire™ 744 (left), or mouse IgG1, κ PE/Fire™ 744 isotype control (right).

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310971

25 tests

108€

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310972

100 tests

280€

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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4⁺ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections², immunofluorescence microscopy³, and spatial biology (IBEX)^8,9.
RRID: AB_3662217 (BioLegend Cat. No. 310971)
AB_3662217 (BioLegend Cat. No. 310972)

Antigen Details

Structure: C-type lectin, type II glycoprotein, 28/32 kD
Distribution: Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
Function: Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type: B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
Gene ID: 969 View all products for this Gene ID
UniProt: View information about CD69 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD69 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD69	FN50	FC,CyTOF®,IHC-F
FITC anti-human CD69	FN50	FC
PE anti-human CD69	FN50	FC
PE/Cyanine5 anti-human CD69	FN50	FC
APC anti-human CD69	FN50	FC
APC/Cyanine7 anti-human CD69	FN50	FC
PE/Cyanine7 anti-human CD69	FN50	FC
Alexa Fluor® 488 anti-human CD69	FN50	FC
Alexa Fluor® 647 anti-human CD69	FN50	FC,SB
Pacific Blue™ anti-human CD69	FN50	FC
Alexa Fluor® 700 anti-human CD69	FN50	FC
Biotin anti-human CD69	FN50	FC
PerCP/Cyanine5.5 anti-human CD69	FN50	FC
PerCP anti-human CD69	FN50	FC
Brilliant Violet 421™ anti-human CD69	FN50	FC
Brilliant Violet 785™ anti-human CD69	FN50	FC
Brilliant Violet 650™ anti-human CD69	FN50	FC
Brilliant Violet 510™ anti-human CD69	FN50	FC
Brilliant Violet 605™ anti-human CD69	FN50	FC
Purified anti-human CD69 (Maxpar® Ready)	FN50	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD69	FN50	FC
Brilliant Violet 711™ anti-human CD69	FN50	FC
APC/Fire™ 750 anti-human CD69	FN50	FC
TotalSeq™-A0146 anti-human CD69	FN50	PG
TotalSeq™-B0146 anti-human CD69	FN50	PG
TotalSeq™-C0146 anti-human CD69	FN50	PG
Brilliant Violet 750™ anti-human CD69	FN50	FC
KIRAVIA Blue 520™ anti-human CD69	FN50	FC
Spark NIR™ 685 anti-human CD69 Antibody	FN50	FC
PE/Fire™ 640 anti-human CD69	FN50	FC
Spark YG™ 581 anti-human CD69	FN50	FC
TotalSeq™-D0146 anti-human CD69	FN50	PG
APC anti-human CD69	FN50	FC
Spark Blue™ 550 anti-human CD69	FN50	FC
PE anti-human CD69	FN50	FC
Spark Red™ 718 anti-human CD69	FN50	FC
GMP PE anti-human CD69	FN50	FC
PE/Fire™ 810 anti-human CD69	FN50	FC
PE/Fire™ 744 anti-human CD69	FN50	FC
Spark PLUS UV395™ anti-human CD69	FN50	FC
Spark Blue™ 574 anti-human CD69 (Flexi-Fluor™)	FN50	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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