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PE/Fire™ 744 anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
S18006K (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
S18006K_PE-Fire-744_-CD127_FC_081224
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD127 (IL-7Rα) (clone S18006K) PE/Fire™ 744 (left) or rat IgG2b, λ PE/Fire™ 744 isotype control (right).
  • S18006K_PE-Fire-744_-CD127_FC_081224
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) APC and anti-mouse CD127 (IL-7Rα) (clone S18006K) PE/Fire™ 744 (left) or rat IgG2b, λ PE/Fire™ 744 isotype control (right).
Compare all formats See PE/Fire™ 744 spectral data
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158217 25 µg 95€
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158218 100 µg 272€
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IL7R-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages.

* PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sudo T, et al. 1993. Proc Natl Acad Sci USA. 90:9125.
  2. Okuno Y, et al. 2002. Proc Natl Acad Sci USA. 99:6246.
  3. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.10.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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