PerCP/Cyanine5.5 anti-human B7-H4 Antibody anti-B7-H4

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Clone: MIH43 (See other available formats)
Regulatory Status: RUO
Other Names: VTCN1, B7h.5
Isotype: Mouse IgG1, κ
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MIH43_PerCPCy5.5_B7H4_Antibody_020314 — Human B7-H4 transfected P815 cells were stained with B7-H4 (clone MIH43) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).

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358110

100 tests

306€

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Description

B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes: Additional reported applications (for the relevant formats) includes immunofluorescence².
RRID: AB_2563122 (BioLegend Cat. No. 358110)

Antigen Details

Structure: Type I transmembrane protein, member of the B7 family, one IgV-like, one IgC-like domain
Distribution: Activated T cells, B cells, monocytes, and dendritic cells; different cancer types including melanoma, renal, gastric, breast and ovarian
Function: Inhibits T cell proliferation, cytokine secretion, and development of cytotoxicity
Cell Type: B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Antigen References: 1. Fauci JM, et al. 2012. Gynecol. Oncol. 127:420.
2. Chen C, et al. 2012. J. Immunother. 35:354.
3. Guo G, et al. 2012. Clin. Rheumatol. 31:271.
4. Arigami T, et al. 2010. J. Surg. Oncol. 102:748.
Gene ID: 79679 View all products for this Gene ID
UniProt: View information about B7-H4 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All B7-H4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human B7-H4	MIH43	FC,IHC-F,ICC
PE anti-human B7-H4	MIH43	FC
PE/Cyanine7 anti-human B7-H4	MIH43	FC
APC anti-human B7-H4	MIH43	FC
PerCP/Cyanine5.5 anti-human B7-H4	MIH43	FC
PE/Dazzle™ 594 anti-human B7-H4	MIH43	FC
TotalSeq™-A0395 anti-human B7-H4	MIH43	PG
TotalSeq™-C0395 anti-human B7-H4	MIH43	PG
APC/Fire™ 750 anti-human B7-H4	MIH43	FC
TotalSeq™-B0395 anti-human B7-H4	MIH43	PG
Brilliant Violet 421™ anti-human B7-H4	MIH43	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 05.05.2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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